Recombinant human Glutathione Reductase protein | NKMAX ATGP1318 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Glutathione Reductase protein

catalog :

ATGP1318

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Glutathione Reductase protein

Domain :

43-522aa

Catalog number (SKU) :

ATGP1318

NCBI :

NP_000628

Description :

Glutathione reductase, also known as GSR, is a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein and plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG) : GSSG + NADPH + H+ ->2GSH + NADP+. In most eukaryotic cells, GSR maintains the ratio of [GSH] / [GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. Recombinant human GSR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glutathione reductase, GLuR, GRD1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Stoll V S., et al. (1997) Biochemistry. 36:6437-6447.;; Karplus P A., et al. (1987) J Mol biol. 195:701-729.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 45unit/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Human

Gene Category :

Redox Proteins

Molecular Weight :

54.3 kDa (504aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 45 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMAMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV PKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE VLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCA NKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00390

Internal Search Terms :

Glutathione Reductase, Glutathione reductase, GLuR, GRD1,ATGP1318-10ug,ATGP1318-20ug,ATGP1318-50ug,ATGP1318-100ug,ATGP1318-250ug,ATGP1318-500ug,ATGP1318-1mg,ATGP1318-10,ATGP1318-20,ATGP1318-50,ATGP1318-100,ATGP1318-250,ATGP1318-500,ATGP1318-1

more info or order :

NKMAX product webpage