Recombinant human PA28 gamma/PSME3 protein | NKMAX ATGP1302 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PA28 gamma/PSME3 protein

catalog :

ATGP1302

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PA28 gamma/PSME3 protein

Domain :

1-254aa

Catalog number (SKU) :

ATGP1302

NCBI :

NP_005780.2

Description :

PSME3 (Proteasome activator complex subunit 3) belongs to the PA28 family. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. Recombinant human PSME3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Proteasome activator subunit 3, 11S regulator complex subunit gamma, REG-gamma, Activator of multicatalytic protease subunit 3, Ki nuclear autoantigen, Proteasome activator 28 subunit gamma, PA28g, PA28gamma, Ki

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wilk S., et al. (2000) Arch. Biochem. Biophys. 383:265-271;; Zhang Z., et al. (2008) EMBO J. 27:852-864

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Ubiquitination

Molecular Weight :

31.7 kDa (274aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P61289

Internal Search Terms :

PA28 Activator g Subunit, Proteasome activator complex subunit 3 isoform 1, Ki, PA28-gamma, PA28G, REG-GAMMA,ATGP1302-10ug,ATGP1302-20ug,ATGP1302-50ug,ATGP1302-100ug,ATGP1302-250ug,ATGP1302-500ug,ATGP1302-1mg,ATGP1302-10,ATGP1302-20,ATGP1302-50,ATGP1302-100,ATGP1302-250,ATGP1302-500,ATGP1302-1

more info or order :

NKMAX product webpage