Recombinant human Isocitrate Dehydrogenase 1/IDH1 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Isocitrate Dehydrogenase 1/IDH1 protein

catalog :

ATGP1284

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Isocitrate Dehydrogenase 1/IDH1 protein

Domain :

1-414aa

Catalog number (SKU) :

ATGP1284

NCBI :

NP_005887.2

Description :

IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. Recombinant human IDH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT,0.1mM PMSF

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Thorsness P.E. et al. (1987) J. Biol. Chem. 262: 10422-10425.;; Geisbrecht B.V. et al. (1999) J. Biol. Chem. 274: 30527-30533.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 18,000pmol/min/ug. One unit will oxidize 1.0pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cancer

Molecular Weight :

48.8 kDa (434aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 18,000 pmol/min/ug. One unit will oxidize 1.0 pmole of DL-Isocitrate to D alpha-Ketoglutarate per minute in the presence of beta-NADP at pH7.4 at 37C.

Amino Acid Sequence :

MSKKISGGSV VEMQGDEMTR IIWELIKEKL IFPYVELDLH SYDLGIENRD ATNDQVTKDA AEAIKKHNVG VKCATITPDE KRVEEFKLKQ MWKSPNGTIR NILGGTVFRE AIICKNIPRL VSGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPSD GTQKVTYLVH NFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKQYKSQFE AQKIWYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLVCPDG KTVEAEAAHG TVTRHYRMYQ KGQETSTNPI ASIFAWTRGL AHRAKLDNNK ELAFFANALE EVSIETIEAG FMTKDLAACI KGLPNVQRSD YLNTFEFMDK LGENLKIKLA QAKL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

O75874

Internal Search Terms :

Isocitrate Dehydrogenase1, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD,ATGP1284-10ug,ATGP1284-20ug,ATGP1284-50ug,ATGP1284-100ug,ATGP1284-250ug,ATGP1284-500ug,ATGP1284-1mg,ATGP1284-10,ATGP1284-20,ATGP1284-50,ATGP1284-100,ATGP1284-250,ATGP1284-500,ATGP1284-1

more info or order :

NKMAX product webpage