product summary
Loading...
company name :
NKMAX
product type :
protein
product name :
Recombinant human eIF-3 alpha/EIF3J protein
catalog :
ATGP1272
quantity :
10 ug
price :
105 USD
more info or order :
NKMAX product webpage
product information
Product Type :
Proteins
Item Name :
Recombinant human eIF-3 alpha/EIF3J protein
Domain :
70-258aa
Catalog number (SKU) :
ATGP1272
NCBI :
NP_003749
Description :
EIF3J (Eukaryotic translation initiation factor 3 subunit J) belongs to the EIF-3 subunit J family. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex. EIF3J binds to the aminoacyl (A) site and mRNA entry channel of the 40S subunit, placing EIF3J directly in the ribosomal decoding center. EIF3J also interacts with eIF1A and reduces 40S subunit affinity for mRNA. A high affinity for mRNA is restored upon recruitment of initiator tRNA, even though EIF3J remains in the mRNA-binding cleft in the presence of tRNA. Recombinant human EIF3J protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names :
Eukaryotic translation initiation factor 3 subunit J, eIF3-alpha, eIF3-p35, EIF3S1, Eukaryotic translation initiation factor 3 subunit 1 alpha
Concentration :
0.5mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Fraser CS, et al. (2007) Mol Cell. 26(6):811-9.;; Block K.L., et al. (1998) J. Biol. Chem. 273:31901-31908
Purity :
> 90% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
Human
Gene Category :
Signal Transduction
Molecular Weight :
24 kDa (210aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Amino Acid Sequence :
MGSSHHHHHH SSGLVPRGSH MKISEKKKIA EKIKEKERQQ KKRQEEIKKR LEEPEEPKVL TPEEQLADKL RLKKLQEESD LELAKETFGV NNAVYGIDAM NPSSRDDFTE FGKLLKDKIT QYEKSLYYAS FLEVLVRDVC ISLEIDDLKK ITNSLTVLCS EKQKQEKQSK AKKKKKGVVP GGGLKATMKD DLADYGGYDG GYVQDYEDFM
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
O75822
Internal Search Terms :
Eukaryotic translation initiation factor 3, Subunit J, subunit 1 alpha, Eukaryotic translation initiation factor 3 subunit J, Eukaryotic translation initiation factor 3 subunit 1 alpha, EIF3S1, eIF3-p35, EIF3J, eIF3-alpha, ATGP1272-10ug, ATGP1272-20ug, ATGP1272-50ug, ATGP1272-100ug, ATGP1272-250ug, ATGP1272-500ug, ATGP1272-1mg, ATGP1272-010, ATGP1272-020, ATGP1272-050, ATGP1272-100, ATGP1272-250, ATGP1272-500, ATGP1272-01M
more info or order :
NKMAX product webpage
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.