Recombinant human RAB32 protein | NKMAX ATGP1246 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human RAB32 protein

catalog :

ATGP1246

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human RAB32 protein

Domain :

1-225aa

Catalog number (SKU) :

ATGP1246

NCBI :

NP_006825

Description :

RAB32 belongs to the small GTPase superfamily. RAB32 modulates ER calcium handling and disrupts the specific enrichment of calnexin on the MAM (mitochondria-associated membrane), while not affecting the ER distribution of protein-disulfide isomerase and mitofusin-2. Also, RAB32 determines the targeting of PKA (cAMP-dependent protein kinase) to mitochondrial and ER membranes and through its overexpression or inactivation increases the phosphorylation of Bad and of Drp1. Through a combination of its functions as a PKA-anchoring protein and a regulator of MAM properties, the activity and expression level of RAB32 determine the speed of apoptosis onset. Recombinant human RAB32 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Ras-related protein Rab-32, RAB32 member RAS oncogene family

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 50% glycerol, 200mM NaCl, 2mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Bui M, et al. (2010) J Biol Chem. 285(41):31590-602.;; Alto NM, et al. (2002) J Cell Biol.158(4):659-68.

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

27.5 kDa (249aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MAGGGA GDPGLGAAAA PAPETREHLF KVLVIGELGV GKTSIIKRYV HQLFSQHYRA TIGVDFALKV LNWDSRTLVR LQLWDIAGQE RFGNMTRVYY KEAVGAFVVF DISRSSTFEA VLKWKSDLDS KVHLPNGSPI PAVLLANKCD QNKDSSQSPS QVDQFCKEHG FAGWFETSAK DNINIEEAAR FLVEKILVNH QSFPNEENDV DKIKLDQETL RAENKSQCC

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q13637

Internal Search Terms :

RAB32, Ras-related protein Rab-32,ATGP1246-10ug,ATGP1246-20ug,ATGP1246-50ug,ATGP1246-100ug,ATGP1246-250ug,ATGP1246-500ug,ATGP1246-1mg,ATGP1246-10,ATGP1246-20,ATGP1246-50,ATGP1246-100,ATGP1246-250,ATGP1246-500,ATGP1246-1

more info or order :

NKMAX product webpage