product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human UbcH5d/UBE2D4 protein
catalog :
ATGP1243
quantity :
20 ug
price :
80 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant human UbcH5d/UBE2D4 protein
Domain :
1-147aa
Catalog number (SKU) :
ATGP1243
NCBI :
NP_057067
Description :
uBE2D4 belongs to the ubiquitin-conjugating enzyme family. ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Recombinant human uBE2D4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names :
Ubiquitin-conjugating enzyme E2 D4, E2 ubiquitin-conjugating enzyme D4, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4
Concentration :
1mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
David Y., et al. (2010) J. Biol. Chem. 285:8595-8604;; Nandi, D, et al. (2006) Journal of biosciences 31 (1): 137-55.
Purity :
> 95% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
Human
Gene Category :
Ubiquitination
Molecular Weight :
18.8 kDa (167aa) confirmed by MALDI-TOF
Amino Acid Sequence :
MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
Q9Y2X8
Internal Search Terms :
UBE2D4, ubiquitin-conjugating enzyme E2 D4, uBCH5D, HBuCE1,ATGP1243-10ug,ATGP1243-20ug,ATGP1243-50ug,ATGP1243-100ug,ATGP1243-250ug,ATGP1243-500ug,ATGP1243-1mg,ATGP1243-10,ATGP1243-20,ATGP1243-50,ATGP1243-100,ATGP1243-250,ATGP1243-500,ATGP1243-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.