Recombinant human VHR/DUSP3 protein | NKMAX ATGP1237 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human VHR/DUSP3 protein

catalog :

ATGP1237

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human VHR/DUSP3 protein

Domain :

1-185aa

Catalog number (SKU) :

ATGP1237

NCBI :

NP_004081

Description :

DuSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DuSP3 is expressed in both breast and ovarian tissues. DuSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Recombinant human DuSP3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Dual specificity protein phosphatase 3, VHR, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, Vaccinia virus phosphatase VH1-related

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Todd JL., et al. (1999) J Biol Chem. 274(19):13271-80.;; Keyse SM., et al. (1995) Biochim Biophys Acta. 1265(2-3):152-60.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 1,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cancer

Molecular Weight :

22.6 kDa (205aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 1,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P51452

Internal Search Terms :

DUSP3, VHR, Dual specificity protein phosphatase 3, VHR,ATGP1237-10ug,ATGP1237-20ug,ATGP1237-50ug,ATGP1237-100ug,ATGP1237-250ug,ATGP1237-500ug,ATGP1237-1mg,ATGP1237-10,ATGP1237-20,ATGP1237-50,ATGP1237-100,ATGP1237-250,ATGP1237-500,ATGP1237-1

more info or order :

NKMAX product webpage