Recombinant human PP1 Catalytic protein | NKMAX ATGP1174 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PP1 Catalytic protein

catalog :

ATGP1174

quantity :

10 ug

price :

240 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PP1 Catalytic protein

Domain :

1-330aa

Catalog number (SKU) :

ATGP1174

NCBI :

NP_002699.1

Description :

PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A

Concentration :

0.2mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 50mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

He B., et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:843-848.;; Trinkle-Mulcahy L., et al. (2001) J. Cell Sci. 114:4219-4228.

Purity :

> 80% by SDS-PAGE

Bioactivity Comment Only :

Specific activity: >3,000unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

39.7 kDa (350aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

Amino Acid Sequence :

MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P62136

Internal Search Terms :

PP1 Catalytic, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A,ATGP1174-10ug,ATGP1174-20ug,ATGP1174-50ug,ATGP1174-100ug,ATGP1174-250ug,ATGP1174-500ug,ATGP1174-1mg,ATGP1174-10,ATGP1174-20,ATGP1174-50,ATGP1174-100,ATGP1174-250,ATGP1174-500,ATGP1174-1

more info or order :

NKMAX product webpage