product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant e.coli rnhA protein
catalog :
ATGP1173
quantity :
20 ug
price :
80 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant e.coli rnhA protein
Domain :
1-155aa
Catalog number (SKU) :
ATGP1173
NCBI :
NP_414750
Description :
rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant e. coli rnhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names :
Ribonuclease HI degrades RNA of DNA-RNA hybrids., Ribonuclease HI, degrades RNA of DNA-RNA hybrids., cer, dasF, ECK0214, herA, JW0204, rnh, sdrA, sin
Concentration :
1mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Nowotny M., et al. (2007) Mol Cell. 28(2):264-76.;; Ten Asbroek AL., et al. (2002) Eur J Biochem. 269(2):583-92.
Purity :
> 95% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
E.coli
Gene Category :
Enzymes & Proteases
Molecular Weight :
20 kDa (178aa) confirmed by MALDI-TOF
Amino Acid Sequence :
MLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P0A7Y4
Internal Search Terms :
rnhA, Ribonuclease HI degrades RNA of DNA-RNA hybrids., Ribonuclease HI, degrades RNA of DNA-RNA hybrids., cer, dasF, ECK0214, herA, JW0204, rnh, sdrA, sin,ATGP1173-10ug,ATGP1173-20ug,ATGP1173-50ug,ATGP1173-100ug,ATGP1173-250ug,ATGP1173-500ug,ATGP1173-1mg,ATGP1173-10,ATGP1173-20,ATGP1173-50,ATGP1173-100,ATGP1173-250,ATGP1173-500,ATGP1173-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.