Recombinant human PPME1 protein | NKMAX ATGP1150 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PPME1 protein

catalog :

ATGP1150

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PPME1 protein

Domain :

1-386aa

Catalog number (SKU) :

ATGP1150

NCBI :

NP_057231

Description :

PPME1, also known protein phosphatase methylesterase-1 (PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile. Recombinant human PPME1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Protein phosphatase methylesterase 1, FLJ22226, PME-1

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ogris E. et al. (1999) J. Biol. Chem. 274: 14382-14391.;; Gagnon S.N. et al. (2002) Biochem. J. 368:263-271.

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

44.4 kDa (406aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI MLIGHSMGGA IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9Y570

Internal Search Terms :

PPME1, Protein phosphatase methylesterase 1, FLJ22226, PME-1,ATGP1150-10ug,ATGP1150-20ug,ATGP1150-50ug,ATGP1150-100ug,ATGP1150-250ug,ATGP1150-500ug,ATGP1150-1mg,ATGP1150-10,ATGP1150-20,ATGP1150-50,ATGP1150-100,ATGP1150-250,ATGP1150-500,ATGP1150-1

more info or order :

NKMAX product webpage