Recombinant human Aldo-keto Reductase 1C4/AKR1C4 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Aldo-keto Reductase 1C4/AKR1C4 protein

catalog :

ATGP1106

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Aldo-keto Reductase 1C4/AKR1C4 protein

Domain :

1-323aa

Catalog number (SKU) :

ATGP1106

NCBI :

NP_001809

Description :

AKR1C4 belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. Recombinant human AKR1C4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Khanna M. et al. (1995) Genomics. 25:588-90.;; Stayrook KR. et al. (2008) Mol Pharmacol. 73:607-612.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

39.2 kDa (343aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P17516

Internal Search Terms :

type I, ST2A3, ST2A1, ST2, member C4, hSTa, HST, HAKRA, dihydrodiol dehydrogenase 4, DHEA-ST, DHEAS, DD4, chlordecone reductase, CHDR, CDR, C11, Aldo-keto Reductase1C4, Aldo-keto reductase family 1 member C4, aldo-keto reductase family 1, AKR1C4, 3-alpha-HSD, 3-alpha hydroxysteroid dehydrogenase type I, 3-alpha hydroxysteroid dehydrogenase, ATGP1106-10ug, ATGP1106-20ug, ATGP1106-50ug, ATGP1106-100ug, ATGP1106-250ug, ATGP1106-500ug, ATGP1106-1mg, ATGP1106-010, ATGP1106-020, ATGP1106-050, ATGP1106-100, ATGP1106-250, ATGP1106-500, ATGP1106-01M

more info or order :

NKMAX product webpage