product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human UCH-L5/UCH37 protein
catalog :
ATGP1069
quantity :
20 ug
price :
80 USD
more info or order :
NKMAX product webpage
product information
Product Type :
Proteins
Item Name :
Recombinant human UCH-L5/UCH37 protein
Domain :
1-329aa
Catalog number (SKU) :
ATGP1069
NCBI :
NP_057068
Description :
ubiquitin carboxyl-terminal hydrolase isozyme L5 (uCHL5) belongs to the peptidase C12 family. This protein is protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Recombinant human uCHL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names :
Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, INO80 complex subunit R, INO80R, UCH37, CGI-70, AD-019
Concentration :
0.5mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 200mM NaCl, 0.1mM PMSF, 2mM EDTA
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Yao T., et al. (2006) Nat. Cell Biol. 8:994-1002;; Nishio K, et al.(2009) Biochem Biophys Res Commun. 390(3):855-60.
Purity :
> 90% by SDS-PAGE
Bioactivity Comment Only :
Specific activity: > 500pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.
Applications :
SDS-PAGE, Enzyme Activity
Antigen Species :
Human
Gene Category :
Ubiquitination
Molecular Weight :
39.7 kDa (349aa) confirmed by MALDI-TOF
Bioactivity Comment and Pics :
Specific activity > 500 pmol/min/ug. Measured by the hydrolysis of ubiquitin-AMC at pH 8.0, at 37C.
Amino Acid Sequence :
MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
Q9Y5K5
Internal Search Terms :
UCH-L5, UCHL5, UCH-37, UCH37, UCH L5, UCH 37, Ubiquitin thioesterase L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin carboxyl-terminal hydrolase isozyme L5, Ubiquitin carboxyl terminal hydrolase isozyme L5, Ubiquitin C terminal hydrolase UCH37, INO80R, INO80 complex subunit R, CGI-70, CGI70, CGI 70, AD-019, AD019, AD 019, ATGP1069-10ug, ATGP1069-20ug, ATGP1069-50ug, ATGP1069-100ug, ATGP1069-250ug, ATGP1069-500ug, ATGP1069-1mg, ATGP1069-010, ATGP1069-020, ATGP1069-050, ATGP1069-100, ATGP1069-250, ATGP1069-500, ATGP1069-01M
more info or order :
NKMAX product webpage
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.