product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant e.coli nth protein
catalog :
ATGP1052
quantity :
10 ug
price :
80 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant e.coli nth protein
Domain :
1-211aa
Catalog number (SKU) :
ATGP1052
NCBI :
NP_416150
Description :
Endonuclease III, also known as nth, is a DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. Recombinant E. coli nth protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Alternative Names :
Endonuclease III, b1633, JW1625, DNA-(apurinic or apyrimidinic site) lyase, Endonuclease-III
Concentration :
0.25mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Cunningham R.P., et al. (1994) Acad. Sci. 726:215-222;; Asahara H., et al. (1989) Biochemistry. 28:4444-4449
Purity :
> 90% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
E.coli
Gene Category :
Enzymes & Proteases
Molecular Weight :
25.7 kDa (231aa) confirmed by MALDI-TOF
Amino Acid Sequence :
MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P0AB83
Internal Search Terms :
nth, Endonuclease III, b1633, JW1625, DNA-(apurinic or apyrimidinic site) lyase, Endonuclease-III,ATGP1052-10ug,ATGP1052-20ug,ATGP1052-50ug,ATGP1052-100ug,ATGP1052-250ug,ATGP1052-500ug,ATGP1052-1mg,ATGP1052-10,ATGP1052-20,ATGP1052-50,ATGP1052-100,ATGP1052-250,ATGP1052-500,ATGP1052-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.