Recombinant e.coli sodA protein | NKMAX ATGP1037 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant e.coli sodA protein

catalog :

ATGP1037

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant e.coli sodA protein

Domain :

1-206aa

Catalog number (SKU) :

ATGP1037

NCBI :

NP_418344

Description :

Superoxide dismutase, Mn, also known as sodA, is a member of the iron/manganese superoxide dismutase family. SodA destroys radicals which are normally produced within the cells and which are toxic to biological systems. It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase. Recombinant E. coli sodA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Superoxide dismutase manganese, Superoxide dismutase, manganese, MnSOD

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Barrister J V., et al. (1987) Crit Rev Biochem. 22:11-180.;; ujika I., et al. (1991) Acta Histochem Cytochem. 24:489-495.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 300unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Redox Proteins

Molecular Weight :

25.2 kDa (226aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 300 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00448

Internal Search Terms :

sodA, Superoxide dismutase manganese, Superoxide dismutase, manganese, MnSOD,ATGP1037-10ug,ATGP1037-20ug,ATGP1037-50ug,ATGP1037-100ug,ATGP1037-250ug,ATGP1037-500ug,ATGP1037-1mg,ATGP1037-10,ATGP1037-20,ATGP1037-50,ATGP1037-100,ATGP1037-250,ATGP1037-500,ATGP1037-1

more info or order :

NKMAX product webpage