product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant e.coli nanA protein
catalog :
ATGP1032
quantity :
20 ug
price :
80 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant e.coli nanA protein
Domain :
1-297aa
Catalog number (SKU) :
ATGP1032
NCBI :
NP_417692
Description :
NanA, also known as N-acetylneuraminate lyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class I aldolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. Recombinant E. coli nanA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Alternative Names :
N-acetylneuraminate lyase, npl
Concentration :
1mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Aisaka K., et al. (1991) Biochem. J. 276:541-546;; Izard T., et al. (1994) Structure 2:361-369
Purity :
> 95% by SDS-PAGE
Applications :
SDS-PAGE
Antigen Species :
E.coli
Gene Category :
Enzymes & Proteases
Molecular Weight :
34.7 kDa (317aa) confirmed by MALDI-TOF
Amino Acid Sequence :
MGSSHHHHHH SSGLVPRGSH MATNLRGVMA ALLTPFDQQQ ALDKASLRRL VQFNIQQGID GLYVGGSTGE AFVQSLSERE QVLEIVAEEA KGKIKLIAHV GCVSTAESQQ LAASAKRYGF DAVSAVTPFY YPFSFEEHCD HYRAIIDSAD GLPMVVYNIP ALSGVKLTLD QINTLVTLPG VGALKQTSGD LYQMEQIRRE HPDLVLYNGY DEIFASGLLA GADGGIGSTY NIMGWRYQGI VKALKEGDIQ TAQKLQTECN KVIDLLIKTG VFRGLKTVLH YMDVVSVPLC RKPFGPVDEK YLPELKALAQ QLMQERG
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P0A6L4
Internal Search Terms :
nanA, N-acetylneuraminate lyase, npl,ATGP1032-10ug,ATGP1032-20ug,ATGP1032-50ug,ATGP1032-100ug,ATGP1032-250ug,ATGP1032-500ug,ATGP1032-1mg,ATGP1032-10,ATGP1032-20,ATGP1032-50,ATGP1032-100,ATGP1032-250,ATGP1032-500,ATGP1032-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.