protein

Recombinant human CD40 Ligand/CD40LG protein

ATGP1017

20 ug

80 USD

Recombinant human CD40 Ligand/CD40LG protein

113-261aa

ATGP1017

NP_000065

CD40 ligand, also known as CD40LG, is a member of the TNF superfamily and is expressed on activated T cells. CD40LG is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. Recombinant human CD40LG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CD40 ligand, CD40-L, CD40L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, hyper-IgM syndrome, HIGM1, T-B cell-activating molecule, T-BAM

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Gordon J., et al. (1995) Blood Rev. 9:53-56.;; Cheng G., et al. (1995) Science. 267:1494-1498.

> 95% by SDS-PAGE

SDS-PAGE

Human

Immunology

18.3 kDa (169aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LCLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL

His-Tag

E.coli

Recombinant Protein

P29965

CD40 Ligand, TNFSF5, CD40 ligand, CD154, CD40L, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP,ATGP1017-10ug,ATGP1017-20ug,ATGP1017-50ug,ATGP1017-100ug,ATGP1017-250ug,ATGP1017-500ug,ATGP1017-1mg,ATGP1017-10,ATGP1017-20,ATGP1017-50,ATGP1017-100,ATGP1017-250,ATGP1017-500,ATGP1017-1