Recombinant human PIN4 protein | NKMAX ATGP0998 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PIN4 protein

catalog :

ATGP0998

quantity :

20 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PIN4 protein

Domain :

1-156aa

Catalog number (SKU) :

ATGP0998

NCBI :

AAH93700

Description :

PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions. Recombinant human PIN4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isofom 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

uchida T., et al. (1999) FEBS Lett. 446:278-282;; Fujiyama-Nakamura S., et al. (2009) Mol. Cell. Proteomics. 8:1552-1565

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

18.8 kDa (176aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLEQFRVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9Y237

Internal Search Terms :

PIN4, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isofom 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17,ATGP0998-10ug,ATGP0998-20ug,ATGP0998-50ug,ATGP0998-100ug,ATGP0998-250ug,ATGP0998-500ug,ATGP0998-1mg,ATGP0998-10,ATGP0998-20,ATGP0998-50,ATGP0998-100,ATGP0998-250,ATGP0998-500,ATGP0998-1

more info or order :

NKMAX product webpage