Recombinant human RDBP/NELFE protein | NKMAX ATGP0974 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human RDBP/NELFE protein

catalog :

ATGP0974

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human RDBP/NELFE protein

Domain :

1-380aa

Catalog number (SKU) :

ATGP0974

NCBI :

NP_002895

Description :

RDBP, also known as NELF-E, is a putative RNA binding protein. This protein is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. RDBP has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions. Recombinant human RDBP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Negative elongation factor complex member E, RD RNA-binding protein, RNA-binding protein RD, RD, D6S45, NELF-E, RDP

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 1mM DTT, 0.1mM PMSF

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wada T., et al. (2000) Mol Cell. 5(6):1067-72.

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

45.4 kDa (400aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Amino Acid Sequence :

MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD KRTQIVYSDD VYKENLVDGF

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P18615

Internal Search Terms :

RDBP, RD RNA binding protein, D6S45, NELF-E, RD, RDP, NELFe,ATGP0974-10ug,ATGP0974-20ug,ATGP0974-50ug,ATGP0974-100ug,ATGP0974-250ug,ATGP0974-500ug,ATGP0974-1mg,ATGP0974-10,ATGP0974-20,ATGP0974-50,ATGP0974-100,ATGP0974-250,ATGP0974-500,ATGP0974-1

more info or order :

NKMAX product webpage