Recombinant human Aldo-keto reductase 1D1/AKR1D1 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Aldo-keto reductase 1D1/AKR1D1 protein

catalog :

ATGP0953

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Aldo-keto reductase 1D1/AKR1D1 protein

Domain :

1-326aa

Catalog number (SKU) :

ATGP0953

NCBI :

NP_005980.1

Description :

Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4) -3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Aldo-keto reductase family 1 member D1, SRD5B1, Delta 4-3-ketosteroid-5-beta-reductase

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Charbonneau A., et al. (2001) Biochim Biophys Acta. 1517:228-235.;; Lemonde H A., et al. (2003) Gut. 52:1494-1499.

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

39.5 kDa (346aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P51857

Internal Search Terms :

Aldo-keto reductase1D1, AKR1D1, Aldo-keto reductase family 1 member D1, Aldo-keto reductase family 1, member D1, 3o5bred, CBAS2, SRD5B1,ATGP0953-10ug,ATGP0953-20ug,ATGP0953-50ug,ATGP0953-100ug,ATGP0953-250ug,ATGP0953-500ug,ATGP0953-1mg,ATGP0953-10,ATGP0953-20,ATGP0953-50,ATGP0953-100,ATGP0953-250,ATGP0953-500,ATGP0953-1

more info or order :

NKMAX product webpage