Recombinant human MASA/ENOPH1 protein | NKMAX ATGP0927 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human MASA/ENOPH1 protein

catalog :

ATGP0927

quantity :

20 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human MASA/ENOPH1 protein

Domain :

1-261aa

Catalog number (SKU) :

ATGP0927

NCBI :

NP_067027

Description :

Enolase-phosphatase E1, also known as ENOPH1, is a member of the MasA family of the HAD (halo-acid dehalogenase) -like hydrolase superfamily. ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an important role in the ubiquitous methionine salvage pathway, a biochemical pathway found in all organisms that regulate methionine levels in the cell. Recombinant human ENOPH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Enolase-phosphatase E1, MASA, MST145, Acireductone synthase, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, E1, mtnC

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhang Y., et al. (2004) Bioorq Med Chem. 12:3847-3855.;; Wang H., et al. (2005) Acta Crystallogr. 61:521-523.

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

31 kDa (281aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT SFSELYLPSS T

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9UHY7

Internal Search Terms :

ENOPH1, Enolase-phosphatase E1, MASA, MST145,ATGP0927-10ug,ATGP0927-20ug,ATGP0927-50ug,ATGP0927-100ug,ATGP0927-250ug,ATGP0927-500ug,ATGP0927-1mg,ATGP0927-10,ATGP0927-20,ATGP0927-50,ATGP0927-100,ATGP0927-250,ATGP0927-500,ATGP0927-1

more info or order :

NKMAX product webpage