Recombinant mouse Dihydrofolate reductase/DHFR protein | NKMAX ATGP0912 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Dihydrofolate reductase/DHFR protein

catalog :

ATGP0912

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Dihydrofolate reductase/DHFR protein

Domain :

1-187aa

Catalog number (SKU) :

ATGP0912

NCBI :

NP_034179.1

Description :

Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX) -resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Dihydrofolate reductase, EC 1.5.1.3

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Chen MJ., et al. (1984) J Biol Chem. 259(6):3933-43. ;; Cody V., et al. (2009) Biochemistry. 48(8):1702-11.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Redox Proteins

Molecular Weight :

23.8 kDa (207aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Amino Acid Sequence :

MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00375

Internal Search Terms :

DHFR, Dihydrofolate reductase, EC 1.5.1.3,ATGP0912-10ug,ATGP0912-20ug,ATGP0912-50ug,ATGP0912-100ug,ATGP0912-250ug,ATGP0912-500ug,ATGP0912-1mg,ATGP0912-10,ATGP0912-20,ATGP0912-50,ATGP0912-100,ATGP0912-250,ATGP0912-500,ATGP0912-1

more info or order :

NKMAX product webpage