protein

Recombinant human eIF-1AY/EIF1AY protein

ATGP0902

10 ug

105 USD

Recombinant human eIF-1AY/EIF1AY protein

1-144aa

ATGP0902

NP_004672

EIF1AY is similar to eukaryotic translation initiation factor 1A (EIF1A). This protein seems to be required for maximal rate of protein biosynthesis. It enhances ribosome dissociation into subunits and is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. Recombinant human EIF1AY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Eukaryotic translation initiation factor 1A Y-linked, Eukaryotic translation initiation factor 1A, Y-linked, eIF-1A Y isoform, eukaryotic translation initiation factor 4C(eIF-4C)

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 100mM NaCl, 20% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Mahbybyl Huq AH., et al. (2009) J Child Neurol. 24(10):1258-61;; Page DC., et al (1997) Science. 278(5338):675-80.

> 95% by SDS-PAGE

SDS-PAGE

Human

Epigenomics (Transcription & Translation)

18.8 kDa (167aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

MGSSHHHHHH SSGLVPRGSH MGSMPKNKGK GGKNRRRGKN ENESEKRELV FKEDGQEYAQ VIKMLGNGRL EALCFDGVKR LCHIRGKLRK KVWINTSDII LVGLRDYQDN KADVILKYNA DEARSLKAYG ELPEHAKINE TDTFGPGDDD EIQFDDIGDD DEDIDDI

His-Tag

E.coli

Recombinant Protein

O14602

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