Recombinant human E2-25K/UBE2K protein | NKMAX ATGP0894 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human E2-25K/UBE2K protein

catalog :

ATGP0894

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human E2-25K/UBE2K protein

Domain :

1-200aa

Catalog number (SKU) :

ATGP0894

NCBI :

NP_005330

Description :

ubiquitin-conjugating enzyme E2 K isoform 1, also known as uBE2K, is a member of the ubiquitin-conjugating enzymes family. These enzymes participate in a variety of cellular processes, including selective protein degradation, DNA repair, cell cycle control, and sporulation. In this mechanism, the ATP-coupled activation and subsequent ligation of ubiquitin are catalyzed by separate enzymes functionally linked by ubiquitin carrier protein uBC1. Also, uBE2K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-beta, huntingtin, and MHC-heavy chain proteins. Recombinant human uBE2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Ubiquitin-conjugating enzyme E2 K isoform 1, E2-25K, HIP2, HYPG, LIG, UBC1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 50mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Chen Z., et al. (1990) J Biol Chem. 264:21835-21742.;; Pichler A., et al. (2005) Nat Struct Mol. 12:264-269.

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Ubiquitination

Molecular Weight :

26.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Amino Acid Sequence :

MANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P61086

Internal Search Terms :

Ubiquitin-conjugating enzyme E2 K isoform 1, Ubiquitin conjugating enzyme E2 K isoform 1, UBE2K, UBC1, UBC 1, LIG, HYPG, HIP2, E2-25K, E225K, E2 25K, ATGP0894-10ug, ATGP0894-20ug, ATGP0894-50ug, ATGP0894-100ug, ATGP0894-250ug, ATGP0894-500ug, ATGP0894-1mg, ATGP0894-010, ATGP0894-020, ATGP0894-050, ATGP0894-100, ATGP0894-250, ATGP0894-500, ATGP0894-01M

more info or order :

NKMAX product webpage