protein

Recombinant human GCA protein

ATGP0886

10 ug

130 USD

Recombinant human GCA protein

1-217aa

ATGP0886

NP_036330.1

GCA, also known as Grancalcin, is calcium-binding protein that is especially abundant in human neutrophils. It belongs to the penta EF-hand (PEF) subfamily of EF-hand proteins, which also comprises calpain, sorcin, peflin, and ALG-2. This protein undergoes important conformational changes upon binding of calcium, which subsequently exposes hydrophobic amino acid residues, which direct the protein to hydrophobic surfaces. It interacts with L-plastin, a protein known to have actin bundling activity, which suggests that GCA may play a role in the regulation of neutrophils adhesion. Recombinant human GCA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Grancalcin, GCL

1mg/ml (determined by BCA assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Teahan C.G., et al. (1992). Biochem. J., 286: 549-554.;; Boyhan A., et al. (1992). J. Biol. Chem., 267: 2928-2933.

> 95% by SDS-PAGE

SDS-PAGE

Human

Signal Transduction

26.1 kDa (237aa) confirmed by MALDI-TOF

MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA LNAWKENFMT VDQDGSGTVE HHELRQAIGL MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA CCVKLRALTD FFRKRDHLQQ GSANFIYDDF LQGTMAI

His-Tag

E.coli

Recombinant Protein

P28676

GCA, Grancalcin, GCL,ATGP0886-10ug,ATGP0886-20ug,ATGP0886-50ug,ATGP0886-100ug,ATGP0886-250ug,ATGP0886-500ug,ATGP0886-1mg,ATGP0886-10,ATGP0886-20,ATGP0886-50,ATGP0886-100,ATGP0886-250,ATGP0886-500,ATGP0886-1

Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012){https://pubmed.ncbi.nlm.nih.gov/22843993/};;Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016){https://pubmed.ncbi.nlm.nih.gov/27040940/};;Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017){https://pubmed.ncbi.nlm.nih.gov/28536714/}