Recombinant human UCHL3 protein | NKMAX ATGP0872 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human UCHL3 protein

catalog :

ATGP0872

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human UCHL3 protein

Domain :

1-230aa

Catalog number (SKU) :

ATGP0872

NCBI :

NP_005993

Description :

ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as uCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. uCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. uCHL3 protein is 54% identical to that of uCHL1. Recombinant human uCHL3 protprot, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

uCH-L3, ubiquitin thioesterase L3, ubiquitin carboxyl-terminal hydrolase isozyme L3, ubiquitin carboxyl-terminal esterase L3

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kwon J., et al. (2007) Exp Anim. 56:71-7.;; Leroy E., et al. (1998) Nature. 395:451-2.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 1,500pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Ubiquitination

Molecular Weight :

28.3 kDa (250aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 1,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P15374

Internal Search Terms :

HUMAN UCHL3, Ubiquitin carboxyl terminal esterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, Ubiquitin carboxyl-terminal esterase L3, Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, Ubiquitin thiolesterase, Ubiquitin thiolesterase L3, UCH L3, UCHL3, UCH-L3, UCHL3 HUMAN, UCHL3_HUMAN, UCHL3HUMAN, ATGP0872-10ug, ATGP0872-20ug, ATGP0872-50ug, ATGP0872-100ug, ATGP0872-250ug, ATGP0872-500ug, ATGP0872-1mg, ATGP0872-010, ATGP0872-020, ATGP0872-050, ATGP0872-100, ATGP0872-250, ATGP0872-500, ATGP0872-01M

more info or order :

NKMAX product webpage