protein

Recombinant human HUS1 protein

ATGP0815

10 ug

240 USD

Recombinant human HUS1 protein

1-280aa

ATGP0815

NP_004498

HuS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex. This protein associates with Rad9 and Rad1 to form the 9-1-1 (RAD9-RAD1-HuS1) complex, which localizes to DNA lesions and promotes DNA damage signaling and repair or apoptosis, cell cycle arrest. The trimeric complex is structurally similar to the proliferating cell nuclear antigen (PCNA) sliding clamp and interacts with Rad17 as a clamp-clamp loader pair during the DNA damage response. Recombinant human HuS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Checkpoint protein HuS1, HUS1 checkpoint clamp component, HUS1 checkpoint homolog, hus1+-like protein, hHUS1

0.25mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 40% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Weiss RS., et al (2009) Proc Natl Acad Sci u S A. 106(50):21282-7.;; Roos-Mattjus P., et al (2002) J Biol Chem. 15;;277(46):43809-12.

> 95% by SDS-PAGE

SDS-PAGE

Human

Cell Cycle

33.8 kDa (300aa) confirmed by MALDI-TOF

MGSSHHHHHH SSGLVPRGSH MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS

His-Tag

E.coli

Recombinant Protein

O60921

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