product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human Glyoxalase II protein
catalog :
ATGP0808
quantity :
10 ug
price :
105 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant human Glyoxalase II protein
Domain :
1-260aa
Catalog number (SKU) :
ATGP0808
NCBI :
NP_001035517
Description :
HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Alternative Names :
Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
Concentration :
0.5mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Vila AJ., et al. (2010) J Inorg Biochem. 104(7):726-31.;; Crowder MW., et al (2009) Biochemistry. 48(23):5426-34.
Purity :
> 95% by SDS-PAGE
Bioactivity Comment Only :
Specific activity is > 500unit/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.
Applications :
Enzyme Activity,SDS-PAGE
Antigen Species :
Human
Gene Category :
Enzymes & Proteases
Molecular Weight :
31.4 kDa (284aa) confirmed by MALDI-TOF
Bioactivity Comment and Pics :
Specific activity is > 500 units/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.
Amino Acid Sequence :
MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
Q16775
Internal Search Terms :
Glyoxalase II, Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1,ATGP0808-10ug,ATGP0808-20ug,ATGP0808-50ug,ATGP0808-100ug,ATGP0808-250ug,ATGP0808-500ug,ATGP0808-1mg,ATGP0808-10,ATGP0808-20,ATGP0808-50,ATGP0808-100,ATGP0808-250,ATGP0808-500,ATGP0808-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.