protein

Recombinant human Sirtuin 2/SIRT2 protein

ATGP0803

10 ug

240 USD

Recombinant human Sirtuin 2/SIRT2 protein

1-352aa

ATGP0803

NP_085096

SIRT2 (NAD-dependent deacetylase sirtuin-2) is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIRT2 is a NAD-dependent deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Recombinant human SIRT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

NAD-dependent protein deacetylase sirtuin-2, NAD-dependent protein defatty-acylase sirtuin-2, Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIR2L, SIR2L2, Sirtuin, Silent mating type information regulation 2 homolog 2 (S. cerevisiae) cerevisiae)

0.25mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 2mM DTT, 200mM NaCl, 0.5mM EDTA

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

North B.J., et al. (2003) Mol. Cell 11:437-444;; Dryden S.C., et al. (2003) Mol. Cell. Biol. 23:3173-3185

> 90% by SDS-PAGE

SDS-PAGE

Human

Cell Cycle

41.6 kDa (372aa) confirmed by MALDI-TOF

MDFLRNLFSQ TLSLGSQKER LLDELTLEGV ARYMQSERCR RVICLVGAGI STSAGIPDFR SPSTGLYDNL EKYHLPYPEA IFEISYFKKH PEPFFALAKE LYPGQFKPTI CHYFMRLLKD KGLLLRCYTQ NIDTLERIAG LEQEDLVEAH GTFYTSHCVS ASCRHEYPLS WMKEKIFSEV TPKCEDCQSL VKPDIVFFGE SLPARFFSCM QSDFLKVDLL LVMGTSLQVQ PFASLISKAP LSTPRLLINK EKAGQSDPFL GMIMGLGGGM DFDSKKAYRD VAWLGECDQG CLALAELLGW KKELEDLVRR EHASIDAQSG AGVPNPSTSA SPKKSPPPAK DEARTTEREK PQ

His-Tag

E.coli

Recombinant Protein

Q8IXJ6

Sirtuin2, SIRT2, NAD-dependent deacetylase sirtuin-2, SIR2, SIR2L, SIR2L2,ATGP0803-10ug,ATGP0803-20ug,ATGP0803-50ug,ATGP0803-100ug,ATGP0803-250ug,ATGP0803-500ug,ATGP0803-1mg,ATGP0803-10,ATGP0803-20,ATGP0803-50,ATGP0803-100,ATGP0803-250,ATGP0803-500,ATGP0803-1

Kawaguchi M, et al. A Fluorescent Probe for Imaging Sirtuin Activity in Living Cells, Based on One-Step Cleavage of the Dabcyl Quencher. (Chembiochem. 2016){https://pubmed.ncbi.nlm.nih.gov/27542094/};;Kawaguchi M, et al. Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin. (J Med Chem. 2019 ){https://pubmed.ncbi.nlm.nih.gov/31117516/}