Recombinant human Purine nucleoside phosphorylase/PNP protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Purine nucleoside phosphorylase/PNP protein

catalog :

ATGP0786

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Purine nucleoside phosphorylase/PNP protein

Domain :

1-289aa

Catalog number (SKU) :

ATGP0786

NCBI :

NP_000261

Description :

PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Alternative Names :

Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PuNP

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Dautant A., et al. (2010) J Biol Chem. 285(38):29502-10.;; Balakrishnan K., et al. (2010) Blood. 116(6):886-92.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 120unit/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.

Applications :

SDS-PAGE,Enzyme Activity

Antigen Species :

Human

Gene Category :

Neuroscience

Molecular Weight :

34.2 kDa (309aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 120 units/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.

Amino Acid Sequence :

MENGYTYEDY KNTAEWLLSH TKHRPQVAII CGSGLGGLTD KLTQAQIFDY GEIPNFPRST VPGHAGRLVF GFLNGRACVM MQGRFHMYEG YPLWKVTFPV RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FSGQNPLRGP NDERFGDRFP AMSDAYDRTM RQRALSTWKQ MGEQRELQEG TYVMVAGPSF ETVAECRVLQ KLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SLEKANHEEV LAAGKQAAQK LEQFVSILMA SIPLPDKAS

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00491

Internal Search Terms :

Purine nucleoside phosphorylase, PNP,ATGP0786-10ug,ATGP0786-20ug,ATGP0786-50ug,ATGP0786-100ug,ATGP0786-250ug,ATGP0786-500ug,ATGP0786-1mg,ATGP0786-10,ATGP0786-20,ATGP0786-50,ATGP0786-100,ATGP0786-250,ATGP0786-500,ATGP0786-1

more info or order :

NKMAX product webpage