Recombinant human NANS protein | NKMAX ATGP0784 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human NANS protein

catalog :

ATGP0784

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human NANS protein

Domain :

1-359aa

Catalog number (SKU) :

ATGP0784

NCBI :

NP_061819

Description :

NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins) -like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Recombinant human NANS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Sialic acid synthase, SAS, N acetylneuraminate synthase

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Milstein O., et al. (2008) J Biol Chem. 283(49):34414-22.;; Aldape MJ., et al. (2007) J Infect Dis. 195(12):1838-45.;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

42.4 kDa (379aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9NR45

Internal Search Terms :

NANS, Sialic acid synthase, SAS, N acetylneuraminate synthase,ATGP0784-10ug,ATGP0784-20ug,ATGP0784-50ug,ATGP0784-100ug,ATGP0784-250ug,ATGP0784-500ug,ATGP0784-1mg,ATGP0784-10,ATGP0784-20,ATGP0784-50,ATGP0784-100,ATGP0784-250,ATGP0784-500,ATGP0784-1

more info or order :

NKMAX product webpage