Recombinant human PMM1 protein | NKMAX ATGP0782 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PMM1 protein

catalog :

ATGP0782

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PMM1 protein

Domain :

1-262aa

Catalog number (SKU) :

ATGP0782

NCBI :

NP_002667

Description :

PMM1 (Phosphomannomutase 1) is an enzyme that involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. This enzyme catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. In addition, it may be responsible for the degradation of glucose-1, 6-bisphosphate in ischemic brain. Recombinant human PMM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

phosphomannomutase 1, Sec53, PMMH22

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl, 0.1mM PMSF

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Veiga-da-Cunha M., et al. (2008) J Biol Chem. 283(49):33988-93.;; Silvaggi N.R., et al. (2006) J. Biol. Chem. 281:14918-14926;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

31.9 kDa (282aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MAVTAQAARR KERVLCLFDV DGTLTPARQK IDPEVAAFLQ KLRSRVQIGV VGGSDYCKIA EQLGDGDEVI EKFDYVFAEN GTVQYKHGRL LSKQTIQNHL GEELLQDLIN FCLSYMALLR LPKKRGTFIE FRNGMLNISP IGRSCTLEER IEFSELDKKE KIREKFVEAL KTEFAGKGLR FSRGGMISFD VFPEGWDKRY CLDSLDQDSF DTIHFFGNET SPGGNDFEIF ADPRTVGHSV VSPQDTVQRC REIFFPETAH EA

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q92871

Internal Search Terms :

PMM1, phosphomannomutase 1, Sec53, PMMH22,ATGP0782-10ug,ATGP0782-20ug,ATGP0782-50ug,ATGP0782-100ug,ATGP0782-250ug,ATGP0782-500ug,ATGP0782-1mg,ATGP0782-10,ATGP0782-20,ATGP0782-50,ATGP0782-100,ATGP0782-250,ATGP0782-500,ATGP0782-1

more info or order :

NKMAX product webpage