protein

Recombinant human Pyridoxal Kinase/PDXK protein

ATGP0777

10 ug

105 USD

Recombinant human Pyridoxal Kinase/PDXK protein

1-312aa

ATGP0777

NP_003672

PDXK converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Recombinant human PDXK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pyridoxal kinase, PKH, PNK, PRED79, Pyridoxine kinase

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Merrill AH Jr., et al. (1984) J Nutr. 114(9):1664-74.;; Hanna M C., et al. (1997) J Biol Chem. 272(16):10756-60.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Metabolism

37.6 kDa (336aa) confirmed by MALDI-TOF

MGSSHHHHHH SSGLVPRGSH MGSHMEEECR VLSIQSHVIR GYVGNRAATF PLQVLGFEID AVNSVQFSNH TGYAHWKGQV LNSDELQELY EGLRLNNMNK YDYVLTGYTR DKSFLAMVVD IVQELKQQNP RLVYVCDPVL GDKWDGEGSM YVPEDLLPVY KEKVVPLADI ITPNQFEAEL LSGRKIHSQE EALRVMDMLH SMGPDTVVIT SSDLPSPQGS NYLIVLGSQR RRNPAGSVVM ERIRMDIRKV DAVFVGTGDL FAAMLLAWTH KHPNNLKVAC EKTVSTLHHV LQRTIQCAKA QAGEGVRPSP MQLELRMVQS KRDIEDPEIV VQATVL

His-Tag

E.coli

Recombinant Protein

O00764

Pyridoxal Kinase, PDXK, Pyridoxal kinase, PKH, PNK, PRED79, Pyridoxine kinase,ATGP0777-10ug,ATGP0777-20ug,ATGP0777-50ug,ATGP0777-100ug,ATGP0777-250ug,ATGP0777-500ug,ATGP0777-1mg,ATGP0777-10,ATGP0777-20,ATGP0777-50,ATGP0777-100,ATGP0777-250,ATGP0777-500,ATGP0777-1

Maity AN, et al. The Nitrogen Atom of Vitamin B6 Is Essential for the Catalysis of Radical Aminomutases. (Int J Mol Sci. 2022){https://pubmed.ncbi.nlm.nih.gov/35563602/}