Recombinant human AK1 protein | NKMAX ATGP0769 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human AK1 protein

catalog :

ATGP0769

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human AK1 protein

Domain :

1-194aa

Catalog number (SKU) :

ATGP0769

NCBI :

NP_000467

Description :

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, Adenylate kinase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Terzic A., et al. (2007) J Biol Chem. 282(43):31366-72.;; Morelli A., et al (2007) Curr Eye Res. 32(3):249-57.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity: > 600unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

23.7 kDa (214aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity > 600 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00568

Internal Search Terms :

AK1, Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase,ATGP0769-10ug,ATGP0769-20ug,ATGP0769-50ug,ATGP0769-100ug,ATGP0769-250ug,ATGP0769-500ug,ATGP0769-1mg,ATGP0769-10,ATGP0769-20,ATGP0769-50,ATGP0769-100,ATGP0769-250,ATGP0769-500,ATGP0769-1

more info or order :

NKMAX product webpage