product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human Bleomycin Hydrolase/BLMH protein
catalog :
ATGP0764
quantity :
20 ug
price :
80 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant human Bleomycin Hydrolase/BLMH protein
Domain :
1-455aa
Catalog number (SKU) :
ATGP0764
NCBI :
NP_000377
Description :
BLMH is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug BLM. It catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Recombinant human BLMH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Alternative Names :
Bleomycin hydrolase, BH, BLM hydrolase, BMH
Concentration :
1mg/ml (determined by Bradford assay)
Formulation :
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Lazo JS.,et al. (2007) Neuroscience. 146(3):890-900;; Abernethy J.,et al (1991) Proc Natl Acad Sci u S A. 96(8):4680-5.
Purity :
> 90% by SDS-PAGE
Bioactivity Comment Only :
Specific activity: > 2,500pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C.
Applications :
SDS-PAGE, Enzyme Activity
Antigen Species :
Human
Gene Category :
Neuroscience
Molecular Weight :
54.7 kDa (475aa) confirmed by MALDI-TOF
Bioactivity Comment and Pics :
Specific activity > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C.
Amino Acid Sequence :
MSSSGLNSEK VAALIQKLNS DPQFVLAQNV GTTHDLLDIC LKRATVQRAQ HVFQHAVPQE GKPITNQKSS GRCWIFSCLN VMRLPFMKKL NIEEFEFSQS YLFFWDKVER CYFFLSAFVD TAQRKEPEDG RLVQFLLMNP ANDGGQWDML VNIVEKYGVI PKKCFPESYT TEATRRMNDI LNHKMREFCI RLRNLVHSGA TKGEISATQD VMMEEIFRVV CICLGNPPET FTWEYRDKDK NYQKIGPITP LEFYREHVKP LFNMEDKICL VNDPRPQHKY NKLYTVEYLS NMVGGRKTLY NNQPIDFLKK MVAASIKDGE AVWFGCDVGK HFNSKLGLSD MNLYDHELVF GVSLKNMNKA ERLTFGESLM THAMTFTAVS EKDDQDGAFT KWRVENSWGE DHGHKGYLCM TDEWFSEYVY EVVVDRKHVP EEVLAVLEQE PIILPAWDPM GALAE
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
Q13867
Internal Search Terms :
Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH, ATGP0764-10ug, ATGP0764-20ug, ATGP0764-50ug, ATGP0764-100ug, ATGP0764-250ug, ATGP0764-500ug, ATGP0764-1mg, ATGP0764-010, ATGP0764-020, ATGP0764-050, ATGP0764-100, ATGP0764-250, ATGP0764-500, ATGP0764-01M
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.