Recombinant human NUDT1/MTH1 protein | NKMAX ATGP0742 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human NUDT1/MTH1 protein

catalog :

ATGP0742

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human NUDT1/MTH1 protein

Domain :

1-156aa

Catalog number (SKU) :

ATGP0742

NCBI :

NP_945191

Description :

NuDT1 is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. It is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. And this protein expressed at much higher levels in proliferating cells than in resting cells. Recombinant human NuDT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Oxidized purine nucleoside triphosphate hydrolase, 2-hydroxy-dATP diphosphatase, 7,8-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, Methylated purine nucleoside triphosphate hydrolase, Nucleoside diphosphate-linked moiety X motif 1 (Nudix motif 1)

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Satoh J., et al (2000) Eur J Neurol. 7(6):673-7.;; Oda H., et al. (1997) J. Biol. Chem. 272:17843-17850;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

20.1 kDa (176aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGASRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAR RELQEESGLT VDALHKVGQI VFEFVGEPEL MDVHVFCTDS IQGTPVESDE MRPCWFQLDQ IPFKDMWPDD SYWFPLLLQK KKFHGYFKFQ GQDTILDYTL REVDTV

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P36639

Internal Search Terms :

7,8-dihydro-8-oxoguanine triphosphatase", 78-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, CD20, CK20, K20, Keratin type I cytoskeletal 20, KRT20, KRT21, MTH1, Nudix motif 1, NUDT1, ATGP0742-10ug, ATGP0742-20ug, ATGP0742-50ug, ATGP0742-100ug, ATGP0742-250ug, ATGP0742-500ug, ATGP0742-1mg, ATGP0742-010, ATGP0742-020, ATGP0742-050, ATGP0742-100, ATGP0742-250, ATGP0742-500, ATGP0742-01M

more info or order :

NKMAX product webpage