Recombinant human CDA protein | NKMAX ATGP0739 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CDA protein

catalog :

ATGP0739

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CDA protein

Domain :

1-146aa

Catalog number (SKU) :

ATGP0739

NCBI :

AAH54036.1

Description :

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2-deoxycytidine for uMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Cytidine deaminase, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase, Cytidine deaminase

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 2mM EDTA, 100mM NaCl, 40% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Evrard A., et al. (2010) Ther Drug Monit. 32(1):53-60.;; Seeberg EC., et a/. (1998) Blood. 91(11):4127-35.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 10,000pmol/min/ug, and is defined as the amount of required to deaminate 1.0pmole of cytidine per min at pH 7.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

18.3 kDa (166aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of required to deaminate 1.0 pmole of cytidine per min at pH 7.5 at 25C.

Amino Acid Sequence :

MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P32320

Internal Search Terms :

CDA, Cytidine deaminase, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase, Cytidine deaminase,ATGP0739-10ug,ATGP0739-20ug,ATGP0739-50ug,ATGP0739-100ug,ATGP0739-250ug,ATGP0739-500ug,ATGP0739-1mg,ATGP0739-10,ATGP0739-20,ATGP0739-50,ATGP0739-100,ATGP0739-250,ATGP0739-500,ATGP0739-1

more info or order :

NKMAX product webpage