Recombinant human DCK protein | NKMAX ATGP0738 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human DCK protein

catalog :

ATGP0738

quantity :

20 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human DCK protein

Domain :

1-260aa

Catalog number (SKU) :

ATGP0738

NCBI :

NP_000779.1

Description :

DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or uTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Deoxycytidine kinase, MGC117410, MGC138632, Deoxycytidine kinase

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.1mM PMSF, 2mM EDTA, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Radu CG., et al. (2010) Proc Natl Acad Sci u S A. 107(12):5551-6;; Ribeiro R., et al. (2007) J Pharmacol Exp Ther. 323(3):935-45;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

34.6 kDa (296aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MATP PKRSCPSFSA SSEGTRIKKI SIEGNIAAGK STFVNILKQL CEDWEVVPEP VARWCNVQST QDEFEELTMS QKNGGNVLQM MYEKPERWSF TFQTYACLSR IRAQLASLNG KLKDAEKPVL FFERSVYSDR YIFASNLYES ECMNETEWTI YQDWHDWMNN QFGQSLELDG IIYLQATPET CLHRIYLRGR NEEQGIPLEY LEKLHYKHES WLLHRTLKTN FDYLQEVPIL TLDVNEDFKD KYESLVEKVK EFLSTL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P27707

Internal Search Terms :

DCK,ATGP0738-10ug,ATGP0738-20ug,ATGP0738-50ug,ATGP0738-100ug,ATGP0738-250ug,ATGP0738-500ug,ATGP0738-1mg,ATGP0738-10,ATGP0738-20,ATGP0738-50,ATGP0738-100,ATGP0738-250,ATGP0738-500,ATGP0738-1

more info or order :

NKMAX product webpage