Recombinant human Selenoprotein R/MSRB1 protein | NKMAX ATGP0735 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Selenoprotein R/MSRB1 protein

catalog :

ATGP0735

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Selenoprotein R/MSRB1 protein

Domain :

1-116aa

Catalog number (SKU) :

ATGP0735

NCBI :

NP_057416

Description :

SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases (MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO. In bacteria, the selenocystein (Sec/u) element is located immediately following the uGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

HSPC270, Methionine-R-sulfoxide reductase B1, MsrB1, Selenoprotein X, SELENOR, SELENOX, SelR, SelX, SepR, SEPX1

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.1mM PMSF, 2mM EDTA, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Gladdyshev VN., et al. (2010) PLoS One. 5(7):e11497.;; Thiele JJ., et al. (2009) Am J Dermatopathol. 31(5):427-31.;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Redox Proteins

Molecular Weight :

14.8 kDa (136aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9NZV6

Internal Search Terms :

SEPX1, Methionine-R-sulfoxide reductase B1, Selenoprotein X, HSPC270, MSRB1, SELR, SELX, MSRB1, Methionine-R-sulfoxide reductase B1, u95C,ATGP0735-10ug,ATGP0735-20ug,ATGP0735-50ug,ATGP0735-100ug,ATGP0735-250ug,ATGP0735-500ug,ATGP0735-1mg,ATGP0735-10,ATGP0735-20,ATGP0735-50,ATGP0735-100,ATGP0735-250,ATGP0735-500,ATGP0735-1

more info or order :

NKMAX product webpage