protein

Recombinant human PD-ECGF/TYMP protein

ATGP0732

20 ug

105 USD

Recombinant human PD-ECGF/TYMP protein

11-482aa

ATGP0732

NP_001107228

Thymidine phosphorylase precursor, also known TYMP, is a platelet-derived endothelial cell growth factor that catalyzes the formation of thymine and 2-deoxy-D-ribose-1-phosphate from thymidine and orthophosphate. It is an angiogenic inducer that potently stimulates the growth of endothelial cells and induces chemotaxis. Increased expression of TYMP is seen in a wide variety of different solid tumors and inflammatory diseases and is often associated with poor prognosis. Recombinant human TYMP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Thymidine phosphorylase, ECGF1, hPD-ECGF, MNGIE, PDECGF, TP, Gliostatin, Platelet-derived endothelial cell growth factor, TdRPase

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Heldin C H., et al. (1991) J Cell Biochem. 47:208-210.;; Furukawa T., et al. (1992) Nature. 356:668.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Growth factors

51.3 kDa (493aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MGSSHHHHHH SSGLVPRGSH MAPPAPGDFS GEGSQGLPDP SPEPKQLPEL IRMKRDGGRL SEADIRGFVA AVVNGSAQGA QIGAMLMAIR LRGMDLEETS VLTQALAQSG QQLEWPEAWR QQLVDKHSTG GVGDKVSLVL APALAACGCK VPMISGRGLG HTGGTLDKLE SIPGFNVIQS PEQMQVLLDQ AGCCIVGQSE QLVPADGILY AARDVTATVD SLPLITASIL SKKLVEGLSA LVVDVKFGGA AVFPNQEQAR ELAKTLVGVG ASLGLRVAAA LTAMDKPLGR CVGHALEVEE ALLCMDGAGP PDLRDLVTTL GGALLWLSGH AGTQAQGAAR VAAALDDGSA LGRFERMLAA QGVDPGLARA LCSGSPAERR QLLPRAREQE ELLAPADGTV ELVRALPLAL VLHELGAGRS RAGEPLRLGV GAELLVDVGQ RLRRGTPWLR VHRDGPALSG PQSRALQEAL VLSDRAPFAA PSPFAELVLP PQQ

His-Tag

E.coli

Recombinant Protein

P19971

PD-ECGF, Thymidine Phosphorylase, TYMP, Thymidine phosphorylase, ECGF1, hPD-ECGF, MNGIE, PDECGF, TP, Gliostatin, Thymidine phosphorylase,ATGP0732-10ug,ATGP0732-20ug,ATGP0732-50ug,ATGP0732-100ug,ATGP0732-250ug,ATGP0732-500ug,ATGP0732-1mg,ATGP0732-10,ATGP0732-20,ATGP0732-50,ATGP0732-100,ATGP0732-250,ATGP0732-500,ATGP0732-1

Matsumae G, et al. Targeting thymidine phosphorylase as a potential therapy for bone loss associated with periprosthetic osteolysis. (BioengTransl Med. 2021){https://pubmed.ncbi.nlm.nih.gov/34589604/}