Recombinant human HIF-1 alpha/HIF1A protein | NKMAX ATGP0730 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human HIF-1 alpha/HIF1A protein

catalog :

ATGP0730

quantity :

10 ug

price :

110 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human HIF-1 alpha/HIF1A protein

Domain :

1-85aa

Catalog number (SKU) :

ATGP0730

NCBI :

NP_001521.1

Description :

Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1 beta subunit and one of three subunits (Hif-1 alpha, Hif-2 alpha or Hif-3 alpha). The activation of Hif-1 alpha is closely associated with a variety of tumors and oncogenic pathways. Hif-1 alpha consists of DNA binding domain (DBD domain), dimerization domain and C-terminal regulatory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Recombinant human Hif-1 alpha (1-85) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Hypoxia inducible factor 1 subunit alpha, Hypoxia-inducible factor 1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, PASD8, MOP1

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.2M NaCl, 1mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Okuyama H., et al. (2006) J Biol Chem. 281(22):15554-63.

Purity :

> 80% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

11.8 kDa (105aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Amino Acid Sequence :

MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q16665

Internal Search Terms :

HIF1 a, Hypoxia-inducible factor 1-alpha, BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha,ATGP0730-10ug,ATGP0730-20ug,ATGP0730-50ug,ATGP0730-100ug,ATGP0730-250ug,ATGP0730-500ug,ATGP0730-1mg,ATGP0730-10,ATGP0730-20,ATGP0730-50,ATGP0730-100,ATGP0730-250,ATGP0730-500,ATGP0730-1

more info or order :

NKMAX product webpage