Recombinant human DUTPase/DUT protein | NKMAX ATGP0725 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human DUTPase/DUT protein

catalog :

ATGP0725

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human DUTPase/DUT protein

Domain :

70-252aa

Catalog number (SKU) :

ATGP0725

NCBI :

NP_001020419

Description :

DuT, also known as duTP pyrophosphatase, is a ubiquitous enzyme that functions in nucleotide metabolism. This protein, in the presence of magnesium ions, is responsible for hydrolyzing duTP to duMP and diphosphate. This reaction is important for keeping the intracellular duTP concentration low so that uracil does not become incorporated into DNA. Extensive incorporation of uracil into DNA can ultimately lead to cell death. This suggests that DuT is essential for cell viability, further implying that it is a potential target for anticancer therapy. Recombinant human DuT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

FLJ20622, duTPase, duTP pyrophosphatase, duTP diphosphatase, Deoxyuridine Triphosphatase, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Mclntosh EM., et al. (1992) Proc Natl Acad Sci u S A. 89(17):8020-4. ;; Persson R., et al. (2001) Curr Protein Pept Sci. 2(4):287-300.;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

21.6 kDa (204aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MASTVGAAGW KGELPKAGGS PAPGPETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P33316

Internal Search Terms :

DuT, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial, duTPase, duTP pyrophosphatase, FLJ20622, Deoxyuridine 5-triphosphate nucleotidohydrolase mitochondrial, Deoxyuridine Triphosphatase,ATGP0725-10ug,ATGP0725-20ug,ATGP0725-50ug,ATGP0725-100ug,ATGP0725-250ug,ATGP0725-500ug,ATGP0725-1mg,ATGP0725-10,ATGP0725-20,ATGP0725-50,ATGP0725-100,ATGP0725-250,ATGP0725-500,ATGP0725-1

more info or order :

NKMAX product webpage