Recombinant human PKLR protein | NKMAX ATGP0714 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PKLR protein

catalog :

ATGP0714

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PKLR protein

Domain :

47-574aa

Catalog number (SKU) :

ATGP0714

NCBI :

NP_000289

Description :

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The L-type isoform also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young. Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Meza NW., et al. (2009) Mol Ther. 17(12):2000-9.;; Roy MF., et al. (2007) J Exp Med. 204(12):2949-61.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity: > 80unit/mg. One unit will convert 1.0 umole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Human

Gene Category :

Cancer

Molecular Weight :

59.2 kDa (549aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity > 80 units/mg. One unit will convert 1.0 umole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P30613

Internal Search Terms :

PKLR, Pyruvate kinase isozymes R/L, PK1, PKL, PKR, PKRL, RPK,ATGP0714-10ug,ATGP0714-20ug,ATGP0714-50ug,ATGP0714-100ug,ATGP0714-250ug,ATGP0714-500ug,ATGP0714-1mg,ATGP0714-10,ATGP0714-20,ATGP0714-50,ATGP0714-100,ATGP0714-250,ATGP0714-500,ATGP0714-1

more info or order :

NKMAX product webpage