protein

Recombinant human ERK1/MAPK3 protein

ATGP0687

20 ug

80 USD

Recombinant human ERK1/MAPK3 protein

1-379aa

ATGP0687

NP_002737

MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This protein is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Recombinant human MAPK3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, PRKM3

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kim DW, et al. (2000) Mol Cell Biol. 20(4):1140-8

> 90% by SDS-PAGE

SDS-PAGE

Human

Cell Cycle

45.2 kDa (399aa) confirmed by MALDI-TOF

MGSSHHHHHH SSGLVPRGSH MAAAAAQGGG GGEPRRTEGV GPGVPGEVEM VKGQPFDVGP RYTQLQYIGE GAYGMVSSAY DHVRKTRVAI KKISPFEHQT YCQRTLREIQ ILLRFRHENV IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS NRPIFPGKHY LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD SKALDLLDRM LTFNPNKRIT VEEALAHPYL EQYYDPTDEP VAEEPFTFAM ELDDLPKERL KELIFQETAR FQPGVLEAP

His-Tag

E.coli

Recombinant Protein

P27361

PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, p44 MAPK, p44 ERK1, Mitogen-activated protein kinase 3, Mitogen activated protein kinase 3, Microtubule-associated protein 2 kinase, Microtubule associated protein 2 kinase, MAPK-3, MAPK3, MAPK 3, MAP kinase3, MAP kinase isoform p44, MAP kinase 3, Insulin-stimulated MAP2 kinase, Insulin stimulated MAP2 kinase, HuMKER1A, HS44KDAP, Extracellular signal-regulated kinase 1, Extracellular signal regulated kinase 1, ERT-2, ERT2, ERT 2, ERK-2, ERK2, ERK-1, ERK1, ERK 2, ERK 1, ATGP0687-10ug, ATGP0687-20ug, ATGP0687-50ug, ATGP0687-100ug, ATGP0687-250ug, ATGP0687-500ug, ATGP0687-1mg, ATGP0687-010, ATGP0687-020, ATGP0687-050, ATGP0687-100, ATGP0687-250, ATGP0687-500, ATGP0687-01M