Recombinant human Malate dehydrogenase 2/MDH2 protein | NKMAX ATGP0679 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Malate dehydrogenase 2/MDH2 protein

catalog :

ATGP0679

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Malate dehydrogenase 2/MDH2 protein

Domain :

25-338aa

Catalog number (SKU) :

ATGP0679

NCBI :

NP_005909

Description :

MDH2 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant human MDH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Malate dehydrogenase 2 NAD (mitochondrial), M-MDH, MDH, MOR1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Thummel CS., et al. (2010) Dev Dyn. 239(3):954-64;; Lee JS., et al (2002) J Basic Microbiol. 42(4):238-45.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 700unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

35.2 kDa (335aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 700 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Amino Acid Sequence :

AKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P40926

Internal Search Terms :

MDH2, Malate dehydrogenase 2 NAD (mitochondrial), M-MDH, MDH, MOR1, Malate dehydrogenase 2, NAD (mitochondrial),ATGP0679-10ug,ATGP0679-20ug,ATGP0679-50ug,ATGP0679-100ug,ATGP0679-250ug,ATGP0679-500ug,ATGP0679-1mg,ATGP0679-10,ATGP0679-20,ATGP0679-50,ATGP0679-100,ATGP0679-250,ATGP0679-500,ATGP0679-1

more info or order :

NKMAX product webpage