protein

Recombinant human OGG1 protein

ATGP0668

20 ug

80 USD

Recombinant human OGG1 protein

1-345aa

ATGP0668

AAH00657.1

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

8-oxoguanine DNA glycosylase, N-glycosylase/DNA lyase, DNA-apurinic or apyrimidinic site lyase, AP lyase, HMMH, HOGG1, OGH1, MUTM

0.5mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Seeberg E., et al. (2002). Nucleic Acids Res. 30(11):2349-57.;; Hodges NJ., et al (2010). DNA Repair (Amst). 9(2):144-52.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Enzymes & Proteases

41.2 kDa (368aa) confirmed by MALDI-TOF

MPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLT QTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVG TKVADCICLM ALDKPQAVPV DVHMWHIAQRDYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRR KGSKGPEG

His-Tag

E.coli

Recombinant Protein

O15527

8-oxoGuanine DNA Glycosylase, OGG1, 8-oxoguanine DNA glycosylase 1, HMMH, HOGG1, MuTM, OGH1, AP lyase, 8-oxoguanine DNA glycosylase 1,ATGP0668-10ug,ATGP0668-20ug,ATGP0668-50ug,ATGP0668-100ug,ATGP0668-250ug,ATGP0668-500ug,ATGP0668-1mg,ATGP0668-10,ATGP0668-20,ATGP0668-50,ATGP0668-100,ATGP0668-250,ATGP0668-500,ATGP0668-1

Duan S, et al. Interaction between RECQL4 and OGG1 promotes repair of oxidative base lesion 8-oxoG and is regulated by SIRT1 deacetylase. (Nucleic Acids Res. 2020){https://pubmed.ncbi.nlm.nih.gov/32432680/};;Schniertshauer D, et al. Real-time Base Excision Repair Assay to Measure the Activity of the 8-oxoguanine DNA Glycosylase 1 in Isolated Mitochondria of Human Skin Fibroblasts. (Bio Protoc. 2021){https://pubmed.ncbi.nlm.nih.gov/33855116/}