protein

Recombinant human Malate dehydrogenase 1/MDH1 protein

ATGP0665

20 ug

80 USD

Recombinant human Malate dehydrogenase 1/MDH1 protein

1-334aa

ATGP0665

NP_005908.1

MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation. Recombinant human MDH1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Malate dehydrogenase cytoplasmic, MDH-s, MDHA, MOR2, Diiodophenylpyruvate reductase

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Youn HD., et al. (2009) Cell Death Differ. 16(5):738-48;; Waye MM., et al (2005) J Cell Biochem. 94(4):763-73.;;

> 95% by SDS-PAGE

Specific activity is > 250unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

SDS-PAGE, Enzyme Activity

Human

Cancer

37.4 kDa (342aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 250 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSA

His-Tag

E.coli

Recombinant Protein

P40925

MOR2, MDH-s, MDHA, MDH1, Malate dehydrogenase cytoplasmic, Malate dehydrogenase, Diiodophenylpyruvate reductase, cytoplasmic, ATGP0665-10ug, ATGP0665-20ug, ATGP0665-50ug, ATGP0665-100ug, ATGP0665-250ug, ATGP0665-500ug, ATGP0665-1mg, ATGP0665-010, ATGP0665-020, ATGP0665-050, ATGP0665-100, ATGP0665-250, ATGP0665-500, ATGP0665-01M

Yoshida T, et al. A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth. (Biochem Biophys Res Commun. 2020){https://pubmed.ncbi.nlm.nih.gov/31787239/}