Recombinant E.coli surA protein | NKMAX ATGP0664 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli surA protein

catalog :

ATGP0664

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli surA protein

Domain :

21-428aa

Catalog number (SKU) :

ATGP0664

NCBI :

NP_414595

Description :

SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E. coli in stationary phase and required for pilus biogenesis. Recombinant E. coli SurA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Chaperone surA, PPIase surA, Rotamase surA, Survival protein A

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Virlogeux-Payant I., et al (2009) Microbiology. 155(Pt 5):1613-22;; Kolter R., et al. (1996) J Bacteriol. 178(6):1770-3.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 800nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

E.coli

Gene Category :

Heat Shock Proteins

Molecular Weight :

47.3 kDa (429aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0ABZ6

Internal Search Terms :

SurA, Chaperone surA, PPIase surA, Rotamase surA, Survival protein A, Chaperone surA,ATGP0664-10ug,ATGP0664-20ug,ATGP0664-50ug,ATGP0664-100ug,ATGP0664-250ug,ATGP0664-500ug,ATGP0664-1mg,ATGP0664-10,ATGP0664-20,ATGP0664-50,ATGP0664-100,ATGP0664-250,ATGP0664-500,ATGP0664-1

more info or order :

NKMAX product webpage