Recombinant E.coli udp protein | NKMAX ATGP0661 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli udp protein

catalog :

ATGP0661

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli udp protein

Domain :

1-253aa

Catalog number (SKU) :

ATGP0661

NCBI :

NP_418275

Description :

uridine phosphorypase (uDP) catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase. Other names in common use include pyrimidine phosphorylase, urdPase, uPH, and uPase. Recombinant E. coli uDP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Uridine phosphorylase, UrdPase, Upase

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Burling F.T., et al. (2003) Acta Crystallogr. 59:73-76;; Walton L., et al (1989) Nucleic Acids Res. 17(16):6741.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 20,000pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

E.coli

Gene Category :

Enzymes & Proteases

Molecular Weight :

29.3 kDa (273aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P12758

Internal Search Terms :

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more info or order :

NKMAX product webpage