Recombinant E.coli nfsB protein | NKMAX ATGP0648 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli nfsB protein

catalog :

ATGP0648

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli nfsB protein

Domain :

1-217aa

Catalog number (SKU) :

ATGP0648

NCBI :

NP_415110

Description :

NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5- (aziridin-1-yl) -2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E. coli NFNB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Oxygen-insensitive NAD(P)H nitroreductase, Dihydropteridine reductase, FMN-dependent nitroreductase, dprA, nfnB

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jarrom D., et al. (2009) Biochemistry. 48(32):7665-72. ;; Singleton DC., et al. (2007) Cancer Gene Ther. 14(12):953-67.;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Redox Proteins

Molecular Weight :

26.0 kDa (237aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P38489

Internal Search Terms :

NFNB, dihydropteridine reductase NAD(P)H-dependent oxygen-insensitive, dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive,ATGP0648-10ug,ATGP0648-20ug,ATGP0648-50ug,ATGP0648-100ug,ATGP0648-250ug,ATGP0648-500ug,ATGP0648-1mg,ATGP0648-10,ATGP0648-20,ATGP0648-50,ATGP0648-100,ATGP0648-250,ATGP0648-500,ATGP0648-1

more info or order :

NKMAX product webpage