Recombinant human HIF-1 alpha/HIF1A protein | NKMAX ATGP0647 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human HIF-1 alpha/HIF1A protein

catalog :

ATGP0647

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human HIF-1 alpha/HIF1A protein

Domain :

576-785aa

Catalog number (SKU) :

ATGP0647

NCBI :

NP_001521

Description :

HIF1A, identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. This protein is a heterodimer composed of HIF-1beta subunit and one of three subunits (HIF-1A, HIF-2A or HIF-3A). The activation of HIF1A is closely associated with a variety of tumors and oncogenic pathways. HIF1A consists of DNA binding domain (DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Recombinant human HIF1A (576-785) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Alternative Names :

Hypoxia inducible factor 1 subunit alpha, Hypoxia-inducible factor 1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, PASD8, MOP1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Okuyama H. et al., (2006) J. Biol Chem. 281(22):15554-63

Purity :

> 85% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Epigenomics (Transcription & Translation)

Molecular Weight :

25.1 kDa (231aa) confirmed by MALDI-TOF (Real Molecular weight on SDS-PAGE will be shift up)

Amino Acid Sequence :

MSFDQLSPLE SSSASPESAS PQSTVTVFQQ TQIQEPTANA TTTTATTDEL KTVTKDRMED IKILIASPSP THIHKETTSA TSSPYRDTQS RTASPNRAGK GVIEQTEKSH PRSPNVLSVA LSQRTTVPEE ELNPKILALQ NAQRKRKMEH DGSLFQAVGI GTLLQQPDDH AATTSLSWKR VKGCKSSEQN GMEQKTIILI PSDLACRLLG Q

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q16665

Internal Search Terms :

HIF1 a, Hypoxia-inducible factor 1-alpha, BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha,ATGP0647-10ug,ATGP0647-20ug,ATGP0647-50ug,ATGP0647-100ug,ATGP0647-250ug,ATGP0647-500ug,ATGP0647-1mg,ATGP0647-10,ATGP0647-20,ATGP0647-50,ATGP0647-100,ATGP0647-250,ATGP0647-500,ATGP0647-1

more info or order :

NKMAX product webpage